Ammonia


                  Ammonia Assay Kit (AMM)

                                         Method: Glutamate Dehydrogenase Method   Liquid reagent, R1: R2=3:1

CLINICAL SIGNIFICANCE

For the in vitro quantitative determination of ammonia in serum or plasma. Under normal circumstances, ammonia converts to urea in the liver. With severe liver disease, ammonia cannot be converted and eliminated from the circulation, which in turn causes blood ammonia increase. High blood ammonia is neurotoxic, and will lead hepatic encephalopathy (hepatic coma). Adult plasma ammonia determination mainly use for monitoring and treatment of hepatic com, while also pediatric diagnosis of Reye's syndrome.


 PRECISION 

Inter assay precision

N=5

Batch 1

Batch 2

Batch 3

Mean(μmol/L)

122.9

121.5

117.3

 

120.57

(Xmax-Xmin)/ 

4.58%


Intra assay precision

N=20

level 1

level 2

Mean(μmol/L)

122.5

439.5

SD

2.18

2.10

CV(%)

1.8%

0.5%


Sensitivity

Sample concentration for 330 μmol/L, the change of absorbance should be not less than 0.0100. 


Correlatio
n

The correlation of Roche and Gcell(y) is y= 1.0686x -0.0341,R2=0.9622


     



Interference

Analytes

Concentration

DB

50mg/dl

Intralipid

100mg/dl

Pyruvic acid

1000mg/dl


Linearity

Linearity is 20-1000umol/L

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