Anti-streptolysin O
Method: Latex Immunoturbidimetric Liquid reagent, R1: R2=3:5
CLINICAL SIGNIFICANCE
Streptolysin O (SLO) is a lethal, exocellular protein produced by Group A streptococci bacteria. It is so named because it is reversibly inactivated by atmospheric oxygen. The binding of active SLO to the surface of erythrocytes causes disruption of the cytoplasmic membrane resulting in cell lysis. Anti-streptolysin O antibodies (ASO) are produced by the host to neutralise the haemolytic action of the SLO. Measurement of ASO in serum is used for the diagnosis ofstreptococcol infections such as rheumatic fever and glomerulonephritis. The ASO level can be used as a measure of the extent and degree of infection. Elevated ASO levels may also be present in other conditions such as scarlet fever, acute rheumatoid arthritis, tonsillitis and various other streptococcal infections and in healthy carriers.
PRECISION
|
Intra assay precision |
||
|
N=20 |
level 1 |
level 2 |
|
Mean(IU/ml) |
142 |
279 |
|
SD |
0.81 |
2.31 |
|
CV(%) |
0.6 |
0.8 |
|
Inter assay precision |
|||
|
N=5 |
Batch 1 |
Batch 2 |
Batch 3 |
|
Mean(IU/ml) |
158 |
153 |
160 |
|
|
157 |
||
|
(Xmax-Xmin)/ |
4.24% |
||
Sensitivity
For analysis sensitivity, the absorbance change rate (△A/min) should be [0.0700～0.4000] under the concentration of 500IU/mL.
Correlation
The correlation of a well-known brand(X) and Gcell(Y) is y = 1.0358x - 4.0196, R2 = 0.9899 and Gcell(Y) is y = 1.0358x - 4.0196, R2 = 0.9899.
Interference
|
Analytes |
Concentration |
|
Bilirubin |
50 mg/dl |
|
Hemoglobin |
500 mg/dl |
|
Intralipid |
600 mg/dl |
|
RF |
380IU/ml |
Linearity
Linearity is 30-1200 IU/mL