NGAL


            Neutrophil Gelatinase-Associated Lipocalin Assay Kit

                                                  Method: Latex Enhanced IT            Liquid reagent, R1: R2=3:1

CLINICAL SIGNIFICANCE

NGAL (Neutrophil Gelatinase-Associated Lipocalin), a 25-kDa glycoprotein covalently bound to gelatinase, it is synthesized and secreted by tubular epithelial cells of the proximal and distal segment. In healthy kidneys, it is barely detectable in either plasma or urine. However, in the setting of acute tubular injury, NGAL undergoes rapid and profound up regulation with large increases in both urine and plasma. Distinct from traditional markers of function such as creatinine, this rapid response enables NGAL to identify injured kidney much earlier than was previously possible.

 

PRECISION 

Intra assay precision

N=20

level 1

level 2

Mean(ng/ml)

151.06

505.64

SD

5.13

8.66

CV(%)

3.39%

1.71%

Inter assay precision

N=5

Batch 1

Batch 2

Batch 3

Mean(ng/ml)

154.30

149.09

152.82

 

152.07

(Xmax-Xmin)/ 

3.43%


Sensitivity

The limit of quantitation (lowest concentration for which CV is less than a target of 20%)is 13.28ng/ml.

Correlation

 The correlation of a well-known brand(X) and Gcell(y) is y = 1.0044x +8.3600, R2 = 0.99814.



     

Interference

Analytes

Concentration

Hemoglobin

500 mg/dl

Vitamin C

50 mg/dl

Direct Bilirubin

30 mg/dl

Heparin

500U/ml

Intralipid

1500mg/dl


Linearity

Linearity  is up to 4920 ng/ml



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