Unsaturated Iron-Bonding Capacity
UIBC
Method: Ferene Liquid reagent: 4:1
CLINICAL SIGNIFICANCE
A known ferrous ion concentration incubated with sample, binds specifically with transferrin at unsaturated iron binding sites. Remaining unbound ferrous ions are measured with the ferene reaction.
The blue complexs (600nm) was formed after the action of the chromogenic agent, and the unsaturated iron-binding force of the serum could be calculated by calculating the reduction iron ions in the buffer solution.
PRECISION
|
Repeatability |
||
|
N=20 |
Level1 |
Level 2 |
|
Mean |
33.28 |
61.90 |
|
SD |
0.62 |
0.55 |
|
CV% |
1.87 |
0.89 |
Total precision
N=20
Level1
Level 2
ST^2
0.78
1.04
ST
0.88
1.02
CV%T
2.72
1.68
SENSITIVITY
The LOD of BSBE UIBC is 3.8 μmol/L.
CORRELATION
The correlation of a well-known brand(Y) and Gcell(X) is y = 1.0287x - 0.0737, R2 = 0.9946.
|
Analytes |
Concentration |
|
HB |
500mg/dl |
|
Ascorbic acid |
50mg/dl |
|
DB |
70mg/dl |
|
Intralipid |
600mg/dl |
|
Zn2+ |
3.0mmol/L |
|
Cu2+ |
3.0mmol/L |
|
Heparin Sodium |
120U/ml |
LINEARITY
The BSBE UIBC linearity is up to 140 μmol/L.