Fructosamine
Method: NBT Method Liquid
reagent, R1: R2=3:1
CLINICAL SIGNIFICANCE
The fructosamine are formed in blood from
glucose present therein. The carbonyl group of the glucose reacts with free
protein amino residues causing the formation of Schiff's base. The half life
time of the fructosamine is 17-20 days. So fructosamine determination is
suitable for a long-term (1-3 weeks) monitoring of sugar metabolism for
patients with diabetes, especially with type Ⅱ diabetes mellitus, and
also suitable for drug efficacy monitoring.
PRECISION
|
Intra assay precision |
||
|
N=20 |
level 1 |
level 2 |
|
Mean (µmol/L) |
333.3 |
389.2 |
|
SD |
1.57 |
1.93 |
|
CV (%) |
0.47% |
0.50% |
Sensitivity
For analysis sensitivity, the absorbance change rate (△A/min) should more than 0.0200 under sample concentration of 290µmol/L.
Correlation
The correlation of a well-known brand(X) and Gcell(Y) is y = 0.7729x + 62.756, R² = 0.8439
Interference
|
Analytes |
Concentration |
|
Direct Bilirubin |
30 mg/dl |
|
Hemoglobin |
2000 mg/dl |
|
Intralipid |
667 mg/dl |
|
VC |
10mg/dl |
|
Glucose |
50 mmol/l |
Linearity
Linearity up to 1000μmol/L.