Lambda Light Chain
Lambda
Method: Immunoturbidimetric Liquid reagent: R1: R2=3:1
There are two types of light chains,kappa and lambda. Each plasma cell synthesizes only one type of heavy chain and only one type of light chain. Heavy and
light chains are then assembled to form immunoglobulins. The rest 40% light chains exist in the blood stream as free light chains. Therefore, the free lifght chains can be tested. Under normal conditions, total κ/λ ratio should be normally around 2:1 in serum. In pathological conditions such as monoclonal gammopathies monoclonal immunoglobulin light chains would change the κ/λ ratio. κ/λratio increasing can be seen in rheumatoid arthritis, systemic lupus erythematosus, acute and chronic hepatitis and cirrhosis, childhood viral encephalitis, primary Sjogren's syndrome, autoimmune diseases, infections, liver disease and kidney disease. κ/λ ratio decreased can be seen in low immunoglobulinemia.
PRECISION
The CV of the test should be CV ≤10%.
|
Serum Precision |
|
GQ-1 |
GQ-2 |
|
NO. of Data Points |
|
20 |
20 |
|
Mean( g/L ) |
|
1.12 |
1.79 |
|
Within-Run |
SD |
0.1 |
0.1 |
|
(Sr) |
CV |
0.5% |
0.6% |
|
Within-Laboratory |
SD |
0.12 |
0.13 |
|
(ST) |
CV |
1.54% |
1.03% |
SENSITIVITY
When the sample concentration is 1.75 g/L, the change in absorbance should be more than 0.1000.
CORRELATION
The correlation of a well-known brand(X) and Gcell(Y) is y = 1.0497x - 0.0579, R2 = 0.9976.
|
Analytes |
Concentration |
|
Hemoglobin |
500mg/dL |
|
Intralipid |
500mgdL |
|
Bilirubin |
60mg/dL |
|
RF |
300IU/mL |
LINEARITY
Linearity is [0.30, 7.00]g/L.