Von Willebrand Factor Antigen
INTENDED USE
For in vitro quantitative determination of vWF Ag in Plasma.
CLINICAL SIGNIFICANCE
Von Willebrand Factor (vWF) is a glycoprotein synthesized by vascular endothelial cells and bone marrow megakaryocytes. It plays an important role in both the first and second phases of hemostasis. The lack of vWF will cause the patient to become vascular. Hemophilia (von Willebrand disease, vWD), and the overexpression of vWF is of great significance in thrombosis [1]. vWD is a common clinical hereditary bleeding disorder, divided into 3 types, type 1 is caused by partial loss of vWF; type 2 is caused by abnormal quality of vWF, and type 3 is mainly caused by complete lack of vWF. cause. Type 2 vWD is further divided into 4 subtypes. Type 2A vWD is due to the lack of selectivity of high molecular weight vWF multimers, which leads to the weakening of its dependent platelet adhesion function; Type 2B vWD is due to mutations in the vWF gene, which enhances vWF and platelets. GPIb affinity; vWF-dependent platelet adhesion function in 2M type vWD decreases, but the distribution of large and medium molecular weight vWF multimers is normal; 2N type vWD is due to the gene mutation of vWF, resulting in a decrease in the affinity of vWF to FVIII [2]. The reduction of vWF is mainly seen in vWD and acquired von Willebrand disease (AvWD), which is a bleeding disease caused by the production of vWF autoantibodies or excessive vWF clearance due to various acquired factors, and it is mostly related to lymphocyte proliferation. Related diseases (LPD), myeloproliferative diseases (MPD), malignant tumors or autoimmune diseases [3]. In addition, vWF can be used as a specific marker of vascular endothelial dysfunction. The increase can be seen in coronary heart disease, acute coronary syndrome[4], lung cancer[5], hypertension, cerebral infarction[6], liver disease[7] and other diseases .
INTERFERENCE
The effect of bilirubin ≤20mg/dL, hemoglobin ≤ 100 mg/dL, Intralipid≤0.3%,RF≤20IU/mL, Heparin(UFH/LMWH)≤2IU/mL is less than 10%.
ACCURACY
The kit was tested with NIBSC 07/316 international reference material. The deviation of the results should ≤15%.
REPEATABILITY
Repeatability precision was obtained by testing control or sample for 20 times of repeated measurement and CV should ≤10% .
BATCH DIFFERENCE
Randomly select (40±8)% samples from three batches of kits, and the coefficient of variation (CV) of the results should ≤10%.
LINEARITY
In the range of [10，240] %, the linear correlation coefficient should be r≥ 0.99.