Glucose-6-Phosphate Dehydrogenase

G-6-PD

Method: Glucose-6-Phosphate Substrate           Liquid reagent: R1: R2=3: 1

CLINICAL SIGNIFICANCE

Glutathione(GSH) is the necessary condition of maintaining the stability of hemoglobin and the integrity of red cell membrane. Glutathione reductase itself needs sufficient amount of NADPH. The level of NADPH is kept sufficient by G6PD. G6PD deficiency can be triggered by some drugs, such as Antimalarial drugs,  sulfone drugs, primaquine, sulfa drugs. Low G6PD values are found with G6PD deficiency, certain drugs, fava beans, infections, etc.
PRECISION 

Inter assay precision

N=5

Batch 1

Batch 2

Batch 3

Mean(U/L)

781.40

753.00

783.00


(U/L)


772.47

CV(%)=(Xmax-

Xmin)/

3.75%


Intra assay precision

N=20

Sample 1

Sample 2

Mean( U/L)

797.10

2714.5

SD

10.4

10.52

CV(%)

1.3%

0.39%

SENSITIVY

For analysis sensitivity, the absorbance change rate (△A/min) should be 0.0070 under the concentration of  2500 U/L.

CORRELATION

Tested the blood samples with Gcell G-6-PD assay kit (X) and a well-known brand kit (Y) at the same time. The correlation formula is Y=0.9897X- 56.045, R² = 0.9892.

LINEARITY

Linearity is 506000 U/L.

 

 

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