Glucose-6-Phosphate Dehydrogenase
G-6-PD
Method: Glucose-6-Phosphate Substrate Liquid reagent: R1: R2=3: 1
CLINICAL SIGNIFICANCE
Glutathione(GSH) is the necessary condition of maintaining the stability of hemoglobin and the integrity of red cell membrane. Glutathione reductase itself needs sufficient amount of NADPH. The level of NADPH is kept sufficient by G6PD. G6PD deficiency can be triggered by some drugs, such as Antimalarial drugs, sulfone drugs, primaquine, sulfa drugs. Low G6PD values are found with G6PD deficiency, certain drugs, fava beans, infections, etc.
PRECISION
Inter assay precision |
|||
N=5 |
Batch 1 |
Batch 2 |
Batch 3 |
Mean(U/L) |
781.40 |
753.00 |
783.00 |
(U/L)
|
772.47 |
||
CV(%)=(Xmax-
Xmin)/ |
3.75% |
Intra assay precision |
||
N=20 |
Sample 1 |
Sample 2 |
Mean( U/L) |
797.10 |
2714.5 |
SD |
10.4 |
10.52 |
CV(%) |
1.3% |
0.39% |
SENSITIVY
For analysis sensitivity, the absorbance change rate (△A/min) should be ≥0.0070 under the concentration of 2500 U/L.
CORRELATION
Tested the blood samples with Gcell G-6-PD assay kit (X) and a well-known brand kit (Y) at the same time. The correlation formula is Y=0.9897X- 56.045, R² = 0.9892.
LINEARITY
Linearity is 50~6000 U/L.