Cortisol
CLINICAL SIGNIFICANCE
Cortisol, also known as hydrocortisone, is the main glucocorticoid secreted from the adrenal cortex. Cortisol plays an important role in the regulation of many important physiological processes, including energy metabolism, maintaining electrolyte and blood pressure balance, immune regulation, stress response, cell proliferation and differentiation, memory regulation and cognitive function. Cortisol in the blood is mainly combined with corticosteroid-binding globulin and albumin, free cortisol only accounts for 3-5%, and the cortisol concentration level changes periodically every day, reaching the lowest in the first half of the night, and reaching the peak in the early morning. The determination of cortisol in the blood is mainly used for human diseases such as excessive cortisol secretion caused by Cushing’s syndrome and cortisol deficiency caused by Addison’s disease, and treatment monitoring (dexamethasone inhibition Therapy and hormone replacement therapy).
SENSITIVITY
SENSITIVITY
When the sample concentration is 200 ng/mL, the change of absorbance should ≥ 0.0200.
PRECISION
Repeatability precision
Use samples with high and low concentrations to repeat the measurement 20 times, and the coefficient of variation (CV%) of the measured value should not be more than 10.0%.
LINEARITY
The linearity is [20, 750.0] ng/mL.
INTERFERENCE
LINEARITY
The linearity is [20, 750.0] ng/mL.
INTERFERENCE
|
Intralipid |
up to 0.5% |
|
DBIL |
up to 20 mg/dl |
|
Hemoglobin |
up to 500mg/dl |
|
RF |
up to 500 IU/mL |