Lipoprotein-associated Phospholipase A2
CLINICAL SIGNIFICANCE
Lp-PLA2 is a calcium-independent member of the growing phospholipase A2 superfamily. It is produced by inflammatory cells, and hydrolyzes oxidized phospholipids. It is predominantly asscoiated with low-density lipoprotein(LDL) and, to a lesser extent, .high-density lipoprotein(HDL) in human serum and plasma. Lp-PLA2 hydrolyzes oxidized LDL, generating lysophosphatidylcholine (Lyso-PC) and oxidized fatty acids.Both of the products of Lp-PLA2 hydrolysis are pro-inflammatory and pro-atherogenic mediators, and play important roles in the pathophysiology of cardiovascular disease. Lp-PLA2 could be used as biomarker of cardiovascular disease. Unlike C-reactive protein (CRP), Lp - PLA2 is a high specific marker of vascular inflammation. Clinical studies have reported that Lp-PLA2 activity is strongly associated with atherogenic and vascular risk. By monitoring the activity of the Lp-PLA2, it will be efficacious in predicting and decreasing the risk of atherosclerosis.
SENSITIVITY
SENSITIVITY
When the sample concentration is 200.0U/L, the absorbance change should ≥0.0050.
PRECISION
CORRELATION
LINEARITY
The linearity is [50.0, 1200.0] U/L.
INTERFERENCES
PRECISION
a）Repeatability Precision（N=20）
|
|
Mean (U/L) |
CV(%) |
|
Control 1 |
280.515 |
0.66 |
|
Control 2 |
754.955 |
0.52 |
b) Intermediate Precision (N=80)
|
|
Mean (U/L) |
CV(%) |
|
Control 1 |
386.44 |
1.64 |
|
Control 2 |
730.81 |
1.54 |
CORRELATION
The correlation of a well-known brand(x) and Gcell(y) is y = 1.013x-7.2954 R2=0.9987
LINEARITY
The linearity is [50.0, 1200.0] U/L.
INTERFERENCES
|
Bilirubin |
up to 50mg/dL |
|
Hemoglobin |
up to 1000 mg/dL |
|
Ascorbic acid |
up tp 60mg/dL |
|
Intralipid |
up to 2000 mg/dL |